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KMID : 0361619740090010091
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Journal of the Korean Orthopaedic Association
1974 Volume.9 No. 1 p.91 ~ p.95
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The N.B.T. Dye Reduction Test in Orthopedic Infections
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李錫玄/Lee, Suck-Hyun
朴健寧/韓文植/崔玲姬/金相仁/Park, Kun-Young/Hahn, Moon-Sik/Choi, Young-Hee/Kim, Sang-In
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Abstract
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By various enzymatic activities, colorless Nitroblue-Tetrazolium salts are reduced to form Forma zone which can easily be recognized as dark blue deposits.
Bacterial presence in circulating blood could induce metabolic changes~n neutrophils those are sufficient to cause apon9aneous in-vitro reduction of N. B. T. dye. Increased proportion of Formazan-containing neutrophils in their cytoplasm (NBT-positive neutrophil) would be observed in the course of natural-infection if the involved individual has normal phagocytic activity.
It is the very hypothesis from which Park et al (1968) -devised a new supravital staining technique (NBT test) to differentiate patients with bacterial. infection from without.
Present study was undertaken to apprecia the clinical usefulness of this test by practical appli cation on 54 in-patients in Department of Orthopedic Surgery, Seoul National University Hospital during the period from May 1 to July 31, 1973.
The following observations were obtained;
1. A significant increase of Percentage of NBT (＋) neutrophils was notified in pyogenic ostemyelitia to 23.68.2% CM+SD) for acute and 16.512.8% for chronic respectively, when compared to normal healthy controls which were 5.534 60% for male and . 254.04% for female. -
2. A remarkable increase to 40.316.9% was notified in fresh tuberculosis of bone and joint, whereas old tuberculosis remained in quite normal range by 5.02.8%.
3. A meaningful increase to 19.09.9% was notified also for soft tissue infections.
4. Non-infectious diseases, orthopedical, did show minimal increase to 10. 23.7% which was proved to be negligible by bio-statistical treatment.
5. With respects to above results, the NBT test could darely be said to be a new; simple and 4 reliable teat also for orthopedic infectious conditions.
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KEYWORD
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